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Description:
These expression vectors were generated by replacing the EcoRI-SpeI Dnmt3b insert from the pCAG-Dnmt3b (A609T) construct (a gift from Taiping Chen 1) with a fragment containing multiple cloning sites (MCS). Each plasmid carries one of the mouse Tet genes encoding the full-length protein as described in the publication2 .
Multiple Cloning Sites (MCS)£º
Cloning sites:
EcoRV ¨CAscI-ATG-6XHis-Flag-HA-BamHI-Tet-TGA-NotI BglII (The start and stop codons are underlined; AscI and NotI are unique sites even after the insertion of a Tet gene)
Inserted fragment (Tet gene):
mouse Tet1: 6120 bp
mouse Tet2: 5739 bp
mouse Tet3: 5142 bp
Use E. coli DH5¦Á for transformation and LB with ampicillin for culture. The cloned Tet is tagged with 6xHis, Flag and HA epitopes. The CAG promoter supports expression in 293T cells.
Reference:
1. Chen, T. et al., Mol. Cell. Biol. 23: 5594-5605, 2003
2. He, et al. Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in
Mammalian DNA. Science 333: 1303-1307, 2011.
This product is for research use. No claim or representation is intended for its use to provide information for the diagnosis, prevention, or treatment of a disease
Order information:
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Part # |
Product Name |
Quantity |
Price |
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P 11091 |
pCAG-IRESblast-Tet1 |
3 ¦Ìg |
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|
P 11092 |
pCAG-IRESblast-Tet2 |
|
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|
P 11093 |
pCAG-IRESblast-Tet3 |
|
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